| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kassirer, Natalie G (11)
|
NITWST |
32.94 |
32.83 |
-0.11 |
| 2 |
Drozdowski, Jessica L (12)
|
NITWST |
34.41 |
32.87 |
-1.54 |
| 3 |
Krumpek, Ella E (11)
|
NITWST |
35.56 |
35.17 |
-0.39 |
| 4 |
Scott, Elizabeth M (12)
|
NITWST |
|
36.86 |
- |
| 5 |
Daley, Lily K (12)
|
NITWST |
37.36 |
37.07 |
-0.29 |
| 6 |
Walters, Elizabeth J (11)
|
NITWST |
41.38 |
38.91 |
-2.47 |
| 7 |
Wittmeyer, Paiden E (12)
|
NIHHSC |
44.03 |
40.14 |
-3.89 |
| 8 |
Noecker, Sophia E (11)
|
NITWST |
40.28 |
40.63 |
0.35 |
| 9 |
Delcamp, Ashlynn R (11)
|
NIHHSC |
47.32 |
44.56 |
-2.76 |
| 10 |
Jones, Sophia M (12)
|
NIPVSC |
46.78 |
46.36 |
-0.42 |
| 11 |
Wilson, Gabrielle O (11)
|
NITWST |
54.93 |
47.30 |
-7.63 |
| 12 |
Killelea, Keira m (11)
|
NITTAC |
49.28 |
57.68 |
8.40 |
| 13 |
Shepperd, Sloane A (11)
|
NIEAST |
|
59.52 |
- |
|
Kerins, Bailey R (11)
|
NIHAMB |
52.70 |
DQ |
- |
|
Mancuso, Gabriella J (12)
|
NIHAMB |
51.52 |
NS |
- |